US 11,988,632 B1
Systems for continuous scanning monitoring and analysis based on discrete three-dimensional fluorescence technology
Kai Jiang, Suzhou (CN); Ping Wang, Suzhou (CN); Yawei Tang, Suzhou (CN); and Tao Zhang, Suzhou (CN)
Assigned to SUZHOU HELMEN PRECISION INSTRUMENTS CO., LTD., Suzhou (CN)
Filed by SUZHOU HELMEN PRECISION INSTRUMENTS CO., LTD., Jiangsu (CN)
Filed on Jan. 4, 2024, as Appl. No. 18/404,846.
Claims priority of application No. 202310012417.9 (CN), filed on Jan. 5, 2023.
Int. Cl. G01N 27/44 (2006.01); G01N 27/447 (2006.01)
CPC G01N 27/44721 (2013.01) 7 Claims
OG exemplary drawing
 
1. A system for continuous scanning monitoring and analysis based on discrete three-dimensional fluorescence technology, comprising:
a high-pressure capillary gel electrophoresis mechanism including a plurality of capillary tubes, the plurality of capillary tubes configured to enable passages of different lengths of STR sequence fragments and nucleic acid gene fragments in an energized state;
a detection mechanism configured to be cooperated with the plurality of capillary tubes, including:
a sampling window unit configured to assemble the plurality of capillary tubes and a plurality of detection optical fibers; wherein the sampling window unit includes fixators for fixing the plurality of capillary tubes, and a unique detection optical fiber correspondingly provided at a fixed window position of any one of the plurality of capillary tubes; and a light blocking plate is provided on the fixator between two adjacent capillary tubes;
a detection window unit configured to assemble the plurality of detection optical fibers and a fluorescence signal detection unit; wherein the detection window unit includes a first circular disc for accessing the plurality of detection optical fibers, and a plurality of access channels of the plurality of detection optical fibers are evenly and equidistantly distributed on an outer edge of the first circular disc;
the fluorescence signal detection unit configured to output a plurality of single excitation light sources and obtain fluorescence signals; wherein the fluorescence signal detection unit includes a second circular disc coaxially provided below the first circular disc, the second circular disc is equipped with a plurality of single excitation light source detection modules, an optical detection focus point of each single excitation light source detection module of the plurality of single excitation light source detection modules is cooperated with a center of a circle of an access channel accessed to each of the plurality of detection optical fibers on the first circular disc; a driving mechanism is provided below the second circular disc; and wavelengths of excitation light of any two of the plurality of single excitation light source detection modules are different; and
the plurality of detection optical fibers configured to be cooperated with the sampling window unit and the detection window unit, wherein the plurality of detection optical fibers are shared by the excitation light and an excited light.