	US 11,987,849 B2
	Composition for detecting leptospirosis and method of diagnosing leptospirosis using same
Dong Min Kim, Gwangju (KR); You Mi Lee, Gwangju (KR); and Choon Mee Kim, Gwangju (KR)
Assigned to Industry-Academic Cooperation Foundation, Chosun University, Gwangju (KR)
Filed by INDUSTRY-ACADEMIC COOPERATION FOUNDATION, CHOSUN UNIVERSITY, Gwangju (KR)
Filed on Nov. 15, 2021, as Appl. No. 17/454,980.
Claims priority of application No. 10-2020-0153046 (KR), filed on Nov. 16, 2020.
Prior Publication US 2022/0154251 A1, May 19, 2022
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/686 (2018.01); C12Q 1/689 (2018.01)

CPC C12Q 1/689 (2013.01) [C12Q 1/686 (2013.01); C12Q 2600/112 (2013.01)]

8 Claims

1. A composition for diagnosis of a Leptospira bacterium, the composition comprising a pair of primers of SEQ ID NOS: 1 and 2 and a probe,

wherein the probe comprises SEQ ID NO: 3 and first and second labels,

wherein the first label comprises a fluorescent dye,

wherein the Leptospira bacterium is L. interrogans, and

wherein SEQ ID NOS:1-3 bind specifically to a L. interrogans IS 1500 gene including the nucleotide sequence of SEQ ID NO: 22.