	US 11,987,848 B2
	Fecal sample processing and analysis comprising detection of blood
Joost Louwagie, Dornach (CH)
Assigned to Exact Sciences Corporation, Madison, WI (US)
Filed by Exact Sciences Corporation, Madison, WI (US)
Filed on Mar. 7, 2023, as Appl. No. 18/179,961.
Application 18/179,961 is a continuation of application No. 17/936,335, filed on Sep. 28, 2022, granted, now 11,634,781.
Application 17/936,335 is a continuation of application No. 15/634,607, filed on Jun. 27, 2017.
Application 15/634,607 is a continuation of application No. 15/010,436, filed on Jan. 29, 2016, abandoned.
Application 15/010,436 is a continuation of application No. 13/147,570, abandoned, previously published as PCT/GB2010/000180, filed on Feb. 3, 2010.
Claims priority of provisional application 61/149,581, filed on Feb. 3, 2009.
Prior Publication US 2023/0272488 A1, Aug. 31, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6886 (2018.01); G01N 33/574 (2006.01)

CPC C12Q 1/6886 (2013.01) [G01N 33/57446 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/158 (2013.01); G01N 2333/805 (2013.01); G01N 2800/50 (2013.01); G01N 2800/52 (2013.01)]

18 Claims

1. A method of processing a freshly-collected fecal sample without freezing, the method comprising:

a) collecting a fecal sample from a human subject, wherein the fecal sample is collected at home by the human subject;

b) in a sealable vessel, combining a first portion of the fecal sample with a stabilizing buffer that maintains DNA integrity in a fecal sample, and sealing the sealable vessel; and

c) in a sealable container, combining a second portion of the fecal sample with a solution that prevents denaturation or degradation of blood proteins found in a fecal sample, and sealing the sealable container.