CPC C12Q 1/6886 (2013.01) [C12Q 2600/112 (2013.01); C12Q 2600/154 (2013.01)] | 5 Claims |
1. A method comprising:
extracting genomic DNA from a biological sample of a human individual suspected of having or having colorectal cancer or a large colorectal adenoma;
treating the extracted genomic DNA with bisulfite;
amplifying the bisulfite-treated genomic DNA using primers specific for SFMBT2 and CHST2; and
measuring a methylation level for SFMBT2 and CHST2 using methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, or bisulfite genomic sequencing PCR.
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