US 11,987,845 B2
Methods for tracking improved athletic performance by detecting expression of dysferlin
Matthew Huentelman, Phoenix, AZ (US)
Assigned to The Translational Genomics Research Institute, Phoenix, AZ (US)
Filed by THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE, Phoenix, AZ (US)
Filed on Dec. 18, 2020, as Appl. No. 17/127,968.
Application 17/127,968 is a division of application No. 15/736,296, granted, now 10,889,861, previously published as PCT/US2016/038243, filed on Jun. 17, 2016.
Claims priority of provisional application 62/181,041, filed on Jun. 17, 2015.
Prior Publication US 2021/0115515 A1, Apr. 22, 2021
Int. Cl. C12Q 1/6883 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6876 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6879 (2018.01)
CPC C12Q 1/6883 (2013.01) [C12Q 1/6809 (2013.01); C12Q 1/6876 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6879 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/124 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01); G01N 2800/2807 (2013.01); G01N 2800/52 (2013.01)] 12 Claims
 
1. A method of tracking improved athletic performance in a subject, the method comprising the steps of:
obtaining a set of samples of biofluid from the subject collected before, during and/or after aerobic exercise and stored on sample collection apparatuses, wherein the biofluid is selected from the group consisting of blood, plasma, and serum; the sample collection apparatus comprises a cellulose paper on which the biofluid is placed to dry, and the cellulose paper has not been treated with any chemical stabilizers of nucleic acids;
extracting nucleic acids from the set of samples, wherein the nucleic acids are RNA;
sequencing the extracted nucleic acids to generate sequence data, wherein the RNA is sequenced with RNA-seq; and
analyzing the sequence data to identify increases in gene expression of dysferlin (DYSF), wherein an increase in expression of DYSF in the sample collected during or after aerobic exercise compared to the sample collected before aerobic exercise indicates improved athletic performance;
wherein the sample collected during or after aerobic exercise was obtained from about 1 hour after beginning aerobic exercise to about 4 hours from completion of the aerobic exercise.