US 11,987,839 B2
Compositions of matter for detection assays
Anurup Ganguli, San Diego, CA (US); Ariana Mostafa, San Diego, CA (US); Jacob Berger, San Diego, CA (US); and Ashish Pandey, San Diego, CA (US)
Assigned to VedaBio, Inc., San Diego, CA (US); and The Board of Trustees of The University of Illinois, Urbana, IL (US)
Filed by VedaBio, Inc., San Diego, CA (US); and The Board of Trustees of the University of Illinois, Urbana, IL (US)
Filed on Sep. 24, 2023, as Appl. No. 18/372,098.
Application 18/372,098 is a continuation of application No. 18/208,272, filed on Jun. 10, 2023.
Application 18/208,272 is a continuation of application No. 18/204,337, filed on May 31, 2023, granted, now 11,821,025.
Application 18/204,337 is a continuation of application No. 18/106,420, filed on Feb. 6, 2023, granted, now 11,702,686, issued on Jul. 18, 2023.
Application 18/106,420 is a continuation of application No. 17/861,208, filed on Jul. 9, 2022, granted, now 11,639,520, issued on May 2, 2023.
Claims priority of provisional application 63/289,112, filed on Dec. 13, 2021.
Claims priority of provisional application 63/220,987, filed on Jul. 12, 2021.
Prior Publication US 2024/0026425 A1, Jan. 25, 2024
Int. Cl. C12Q 1/6823 (2018.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/682 (2018.01)
CPC C12Q 1/6823 (2013.01) [C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/682 (2013.01); C12N 2310/20 (2017.05); C12N 2310/315 (2013.01); C12N 2310/321 (2013.01); C12N 2310/3231 (2013.01); C12N 2320/10 (2013.01)] 25 Claims
 
1. A composition of matter comprising:
a ribonucleoprotein complex (RNP) comprising a nucleic acid-guided nuclease and a gRNA complementary to synthesized activating molecules;
a plurality of template molecules comprising a sequence having homology to the gRNA and a primer binding domain;
a plurality of blocked primer molecules comprising a sequence complementary to the primer binding domain of the plurality of template molecules and having a Formula IV, wherein Formula IV in the 5′-to-3′ direction comprises:
T-D-M-A-Lp-C;
wherein T is 17-31 nucleotides in length;
D is 0-15 nucleotides in length;
M is 1-25 nucleotides in length;
A is 0-15 nucleotides in length and comprises a sequence complementary to D; and
L is 3-25 nucleotides in length;
p is 0 or 1;
C is 4-15 nucleotides in length and comprises a sequence complementary to T; and
a polymerase and dNTPs;
wherein when blocked, the blocked primer molecules cannot bind to the template molecules and be extended by the polymerase and wherein when unblocked, the blocked primer molecules can bind to the template molecules and be extended by the polymerase to produce synthesized activating molecules.