US 11,987,834 B2
Acetaminophen adducts and methods of use thereof
Laura P. James, Little Rock, AR (US); Jack Hinson, Little Rock, AR (US); Dean Roberts, Little Rock, AR (US); and Pritmohinder S. Gill, Little Rock, AR (US)
Assigned to BioVentures, LLC, Little Rock, AR (US)
Filed by BioVentures, LLC, Little Rock, AR (US); and Arkansas Children's Hospital Research Institute, Little Rock, AR (US)
Filed on Jun. 6, 2019, as Appl. No. 16/433,657.
Application 16/433,657 is a continuation of application No. 15/118,297, granted, now 10,351,897, issued on Jul. 16, 2019, previously published as PCT/US2015/015905, filed on Feb. 13, 2015.
Claims priority of provisional application 61/940,023, filed on Feb. 14, 2014.
Prior Publication US 2019/0300931 A1, Oct. 3, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/53 (2006.01); C07K 14/47 (2006.01); C12N 9/10 (2006.01); C12Q 1/48 (2006.01); C12Q 1/527 (2006.01)
CPC C12Q 1/48 (2013.01) [C07K 14/4708 (2013.01); C12N 9/1007 (2013.01); C12N 9/1014 (2013.01); C12N 9/107 (2013.01); C12N 9/1096 (2013.01); C12Q 1/527 (2013.01); C12Y 201/01005 (2013.01); G01N 33/53 (2013.01)] 7 Claims
 
1. A method for measuring the amount of an acetaminophen-protein adduct comprising a protein bound to acetaminophen in a biological sample, the method comprising:
a) providing a biological sample from a subject having or suspected of having acetaminophen toxicity;
b) contacting the sample with a first antibody specific to the protein to form a first complex between the first antibody and the protein, wherein the protein is selected from the group consisting of betaine-homocysteine S-methyltransferase 1, cytoplasmic aspartate aminotransferase, 1,4-alpha-glucan-branching enzyme, formimidoyltransferase-cyclodeaminase, and dystrophin;
c) isolating the first complex;
d) contacting the isolated first complex with a second antibody specific to a 3-(cystein-S-yl) APAP (3-Cys-A)-protein linkage on the acetaminophen-protein adduct to form a second complex;
e) measuring the amount of the second complex, wherein the amount of the second complex is indicative of the amount of the acetaminophen-protein adduct.