	US 11,987,830 B2
	Cell-free protein synthesis systems
Nicholas N. Watkins, Livermore, CA (US); Neil Reginald Beer, Livermore, CA (US); and Kenneth W. Turteltaub, Livermore, CA (US)
Assigned to LAWRENCE LIVERMORE NATIONAL SECURITY, LLC, Livermore, CA (US)
Filed by LAWRENCE LIVERMORE NATIONAL SECURITY, LLC, Livermore, CA (US)
Filed on Jan. 24, 2020, as Appl. No. 16/752,222.
Claims priority of provisional application 62/796,427, filed on Jan. 24, 2019.
Prior Publication US 2020/0255881 A1, Aug. 13, 2020
Int. Cl. C12P 21/02 (2006.01); A61K 38/00 (2006.01); B01D 15/38 (2006.01); C12N 11/14 (2006.01); C12N 11/18 (2006.01); C12P 21/00 (2006.01)

CPC C12P 21/02 (2013.01) [B01D 15/3809 (2013.01); C12N 11/18 (2013.01); C12P 21/00 (2013.01); A61K 38/00 (2013.01)]

21 Claims

1. A reusable cell free protein synthesis (CFPS) system for continuously synthesizing protein at a rate of 1.4×10⁻²mg/hr/μL-2.5 mg/hr/μL, the system comprising a plurality of ribosomes attached to a structure, or a plurality of structures, housed, contained, or attached to a solid support,

wherein the solid support comprises a chamber, at least one fluidic inlet, at least one fluidic outlet, and a solution for containing synthesized protein,

wherein the plurality of ribosomes are positioned between the fluidic inlet and the fluidic outlet, and

wherein the solution comprises one or more in vitro translation reagents and synthesized protein moving through the CFPS system and the synthesized protein is collected through the fluidic outlet.