CPC C12P 19/60 (2013.01) [C12N 9/0042 (2013.01); C12N 9/0071 (2013.01); C12N 9/0073 (2013.01); C12N 9/1007 (2013.01); C12N 9/1051 (2013.01); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 9/93 (2013.01); C12Y 106/02004 (2013.01); C12Y 114/11022 (2013.01); C12Y 114/13011 (2013.01); C12Y 114/14 (2013.01); C12Y 204/01 (2013.01); C12Y 204/01185 (2013.01); C12Y 205/01006 (2013.01); C12Y 402/01076 (2013.01); C12Y 403/01023 (2013.01); C12Y 403/01024 (2013.01); C12Y 505/01006 (2013.01); C12Y 602/01012 (2013.01)] | 18 Claims |
1. A recombinant microorganism comprising:
a heterologous nucleic acid sequence coding for a flavanone 7-O-beta-D-glucosyltransferase (UGT) belonging to EC 2.4.1.185, which is capable of adding a glucose in position 7 of hesperetin and/or diosmetin; and
a heterologous nucleic acid sequence coding for a 6″-O-rhamnosyltransferase (RhaT) belonging to EC 2.4.1.653, which is capable of transferring a rhamnose into position 6 of the glucose of hesperetin-7-O-glucoside and/or diosmetin-7-O-glucoside; and
a heterologous nucleic acid sequence coding for a UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase (RHM) belonging to EC 4.2.1.76, which is capable of producing UDP-rhamnose.
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