US 11,987,825 B2
Serine proteases
Marc Kolkman, Oegstgeest (NL); Rie Mejldal, Østbirk (DK); Anja Hemmingsen Kellett-Smith, Århus (DK); and Lilia Maria Babe, Emerald Hills, CA (US)
Assigned to Danisco US Inc., Palo Alto, CA (US)
Filed by DANISCO US INC, Palo Alto, CA (US)
Filed on Jun. 2, 2021, as Appl. No. 17/336,647.
Application 17/336,647 is a continuation of application No. 15/884,749, filed on Jan. 31, 2018, abandoned.
Application 15/884,749 is a continuation of application No. 15/521,386, abandoned, previously published as PCT/US2015/057526, filed on Oct. 27, 2015.
Claims priority of provisional application 62/069,184, filed on Oct. 27, 2014.
Prior Publication US 2021/0395715 A1, Dec. 23, 2021
Int. Cl. C12N 9/64 (2006.01); C11D 3/386 (2006.01); C12N 9/54 (2006.01); C12N 15/70 (2006.01); C12N 15/75 (2006.01); C12N 15/76 (2006.01); C12N 15/77 (2006.01); C12N 15/78 (2006.01)
CPC C12N 9/6424 (2013.01) [C11D 3/386 (2013.01); C12N 9/54 (2013.01); C12N 15/70 (2013.01); C12N 15/75 (2013.01); C12N 15/76 (2013.01); C12N 15/77 (2013.01); C12N 15/78 (2013.01); C12Y 304/21062 (2013.01)] 8 Claims
 
1. A composition comprising a surfactant and a subtilisin comprising a DLGDXXRFGXaGLLXXXXAVX (SEQ ID NO:26) motif, wherein X is any amino acid and Xa is N or S, and wherein said subtilisin comprises an amino acid sequence having at least 85% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 3, and wherein the subtilisin has improved protease activity when compared to protease activity of Bacillus lentus GG36 subtilisin.