	US 11,987,817 B2
	Method of manufacturing cell spheroid using bioink
Hyun Wook Kang, Ulsan (KR); Seug Gyu Jeon, Ulsan (KR); and Jun Ho Heo, Ulsan (KR)
Assigned to UNIST (ULSAN NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY), Ulsan (KR)
Filed by UNIST (ULSAN NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY), Ulsan (KR)
Filed on Jan. 23, 2020, as Appl. No. 16/751,008.
Claims priority of application No. 10-2019-0025956 (KR), filed on Mar. 6, 2019.
Prior Publication US 2020/0283736 A1, Sep. 10, 2020
Int. Cl. C12N 5/071 (2010.01); B29C 64/106 (2017.01); B29K 67/00 (2006.01); B33Y 10/00 (2015.01); B33Y 70/10 (2020.01)

CPC C12N 5/0697 (2013.01) [B29C 64/106 (2017.08); B29K 2005/00 (2013.01); B29K 2067/00 (2013.01); B33Y 10/00 (2014.12); B33Y 70/10 (2020.01); C12N 2502/081 (2013.01); C12N 2502/1323 (2013.01); C12N 2502/14 (2013.01); C12N 2502/22 (2013.01); C12N 2502/28 (2013.01); C12N 2513/00 (2013.01); C12N 2533/54 (2013.01)]

15 Claims

1. A method of manufacturing a cell spheroid, the method comprising:

extruding a first bioink that is composed of a substrate excluding cells and comprising an alginate;

forming a spherical space inside the first bioink by extruding a second bioink comprising dispersed cells into the first bioink;

adding a calcium chloride (CaCl2) solution to the alginate comprised in the first bioink, wherein calcium chloride in the CaCl2 solution is crosslinked with the alginate of the first bioink; and

dissolving the second bioink, present in the first bioink, in a cell culture medium to form a cell spheroid from the cells of the second bioink.