	US 11,987,813 B2
	Microenvironments for self-assembly of islet organoids from stem cells differentiation
Sha Jin, Vestal, NY (US); Kaiming Ye, Vestal, NY (US); and Huanjing Bi, Binghamton, NY (US)
Assigned to The Research Foundation for The Sate University of New York, Binghamton, NY (US)
Filed by The Research Foundation for The State University of New York, Binghamton, NY (US)
Filed on Sep. 7, 2020, as Appl. No. 17/013,830.
Application 17/013,830 is a division of application No. 15/841,004, filed on Dec. 13, 2017, granted, now 10,767,164, issued on Sep. 8, 2020.
Claims priority of provisional application 62/479,095, filed on Mar. 30, 2017.
Prior Publication US 2020/0399611 A1, Dec. 24, 2020
Int. Cl. C12N 5/071 (2010.01); A61L 27/14 (2006.01); A61L 27/36 (2006.01); C12N 5/074 (2010.01); C12N 5/0789 (2010.01)

CPC C12N 5/0677 (2013.01) [A61L 27/14 (2013.01); A61L 27/3633 (2013.01); A61L 27/3695 (2013.01); C12N 5/0647 (2013.01); C12N 5/0696 (2013.01); A61L 2430/34 (2013.01); C12N 2501/117 (2013.01); C12N 2501/16 (2013.01); C12N 2501/599 (2013.01); C12N 2533/54 (2013.01); C12N 2533/90 (2013.01)]

20 Claims

1. A method of creating a pancreatic synthetic organoid, comprising:

(i) extracting a pancreas tissue-specific matrix from a mammalian organ by osmotic processing without a detergent; coating a growth surface with cell growth factors comprising collagen and mucopolysaccharides, and the extracted pancreas tissue-specific matrix;

(ii) culturing pluripotent stem cells on the growth surface; and

(iii) inducing the pluripotent stem cells to differentiate into pancreatic cells on the growth surface.