US 11,987,806 B2
Biocompatible conditioned cell medium compositions and uses thereof
Richard C. Jin, Murrieta, CA (US)
Assigned to Cellum Biomedical, Inc., Irvine, CA (US)
Filed by Cellum Biomedical, Inc., Irvine, CA (US)
Filed on Apr. 22, 2022, as Appl. No. 17/727,323.
Application 17/727,323 is a division of application No. 16/497,177, granted, now 11,345,884, previously published as PCT/US2018/025655, filed on Apr. 2, 2018.
Claims priority of provisional application 62/479,733, filed on Mar. 31, 2017.
Prior Publication US 2022/0325236 A1, Oct. 13, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/00 (2006.01); A61K 9/06 (2006.01); A61K 9/16 (2006.01); A61K 47/36 (2006.01); C12N 5/071 (2010.01); C12N 5/077 (2010.01)
CPC C12N 5/0018 (2013.01) [A61K 9/06 (2013.01); A61K 9/1658 (2013.01); A61K 47/36 (2013.01); C12N 5/0625 (2013.01); C12N 5/0654 (2013.01); C12N 2501/105 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2501/117 (2013.01); C12N 2501/12 (2013.01); C12N 2501/13 (2013.01); C12N 2501/135 (2013.01); C12N 2501/148 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/165 (2013.01); C12N 2501/19 (2013.01); C12N 2501/22 (2013.01); C12N 2501/2301 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/2308 (2013.01); C12N 2501/25 (2013.01); C12N 2502/1142 (2013.01); C12N 2502/1323 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A method of treating a periodontal condition in a human subject, the method comprising administering to a periodontal site in need of a) gum and/or bone growth and/or b) gum erosion and/or bone resorption inhibition in the human subject an effective amount of a biocompatible composition comprising:
(a) a biocompatible polymer matrix, wherein the polymer matrix is agarose; and
(b) a conditioned cell medium comprising i) a cell culture medium and ii) one or more agents synthesized by and secreted from one or more cells cultured in the cell culture medium, wherein the cells are fibroblasts, dermal fibroblasts, neonatal dermal fibroblasts, osteoblasts, fibroblasts and osteoblasts cultured separately, and/or a co-culture of fibroblasts and osteoblasts.