CPC C12N 15/907 (2013.01) [C07K 14/35 (2013.01); C12N 7/00 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12Q 1/70 (2013.01); C12N 2310/20 (2017.05); C12N 2710/16652 (2013.01); C12N 2800/80 (2013.01)] | 44 Claims |
1. A genetically modified mammalian cell comprising a recombination sequence inserted in a target locus on a chromosome of the Vero cell genome, wherein the recombination sequence comprises a Bxb1 attB sequence from Mycobacterium smegmatis wherein the mammalian cell is a Vero cell and the target locus comprises adeno-associated virus integration site 1 (AAVS1) on chromosome 6 of the Vero cell genome, wherein the AAVS1 locus comprising the recombination sequence has the 4 kilobase sequence of SEQ ID NO:22.
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