	US 11,987,803 B2
	Efficient delivery of large cargos into cells on a porous substrate
Ting-Hsiang S. Wu, Culver City, CA (US); Pei-Yu E. Chiou, Los Angeles, CA (US); and Michael A. Teitell, Tarzana, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Oct. 14, 2019, as Appl. No. 16/601,513.
Application 16/601,513 is a continuation of application No. 15/129,387, granted, now 10,472,651, previously published as PCT/US2015/022813, filed on Mar. 26, 2015.
Claims priority of provisional application 61/972,145, filed on Mar. 28, 2014.
Prior Publication US 2020/0199628 A1, Jun. 25, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/89 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 1/42 (2006.01); C12N 15/87 (2006.01)

CPC C12N 15/89 (2013.01) [C12M 25/02 (2013.01); C12M 33/04 (2013.01); C12M 35/00 (2013.01); C12M 35/04 (2013.01); C12N 15/87 (2013.01)]

19 Claims

1. A method of delivering a large cargo into eukaryotic cells, said method comprising:

providing said cells disposed on one side of a porous polymer membrane that comprises a plurality of pores;

providing said cargo suspended in a solution disposed in a reservoir chamber on the opposite side of said porous membrane, wherein said cargo ranges in size from the size of a nucleic acid in a lipid or liposomal complex up to the size of an organelle; and

applying pressure to said reservoir chamber sufficient to pass said cargo through said plurality of pores and through the cell membranes of said cells and into said cells to provide cells transfected with said cargo, wherein said cargo comprises an organelle or a synthetic particle selected from the group consisting of a surface-enhanced Raman scattering (SERS) particle, a quantum dot, and a microbead.