US 11,987,799 B2
Downy mildew resistance in Cucurbitaceae plants
Wim Vriezen, Nunhem (NL); Frank Beenders, Nunhem (NL); and Lieke Mertens, Nunhem (NL)
Assigned to NUNHEMS B.V., Nunhem (NL)
Appl. No. 17/613,401
Filed by Nunhems B.V., Nunhem (NL)
PCT Filed May 18, 2020, PCT No. PCT/EP2020/063809
§ 371(c)(1), (2) Date Nov. 22, 2021,
PCT Pub. No. WO2020/239496, PCT Pub. Date Dec. 3, 2020.
Claims priority of application No. 19176547 (EP), filed on May 24, 2019.
Prior Publication US 2022/0275393 A1, Sep. 1, 2022
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8282 (2013.01) 21 Claims
 
1. A cultivated plant or plant part of the species Cucumis melo comprising at least one copy of a mutant allele of a gene named CmAAP2A, said gene encodes the CmAAP2A protein of SEQ ID NO: 1 or a protein comprising at least 95% sequence identity to SEQ ID NO: 1, wherein said mutant allele results in no expression of the CmAAP2A gene, or wherein the mutant allele encodes a mutant CmAAP2A protein having a loss-of-function compared to the wild type CmAAP2A protein and wherein said mutant protein comprises a mutation in the conserved Amino Acid Transporter Domain at amino acids 20 to 453 of SEQ ID NO: 1 or in an Amino Acid Transporter Domain comprising at least 95% sequence identity to amino acids 20 to 453 of SEQ ID NO: 1, wherein said mutant allele is introgressed from a wild melon or wild relative of melon into the cultivated melon, or wherein said mutant allele is induced by chemical mutagenesis, radiation mutagenesis, tissue culture or targeted genome editing techniques.