US 11,987,797 B2
Attenuated strain of African swine fever virus (ASFV) with IPTG-induced deletion of D1133L gene and use thereof
Haixue Zheng, Lanzhou (CN); Keshan Zhang, Lanzhou (CN); Ting Zhang, Lanzhou (CN); Tao Feng, Lanzhou (CN); Bo Yang, Lanzhou (CN); Xing Yang, Lanzhou (CN); Xijuan Shi, Lanzhou (CN); Hong Tian, Lanzhou (CN); Yi Ru, Lanzhou (CN); Fan Yang, Lanzhou (CN); Zixiang Zhu, Lanzhou (CN); Jianhong Guo, Lanzhou (CN); Jijun He, Lanzhou (CN); and Xiangtao Liu, Lanzhou (CN)
Assigned to LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES, Lanzhou (CN)
Filed by LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES, Lanzhou (CN)
Filed on Nov. 21, 2022, as Appl. No. 18/057,588.
Claims priority of application No. 202210258522.6 (CN), filed on Mar. 16, 2022.
Prior Publication US 2023/0295641 A1, Sep. 21, 2023
Int. Cl. C12N 15/65 (2006.01); C12N 7/00 (2006.01); C12N 15/63 (2006.01)
CPC C12N 15/65 (2013.01) [C12N 7/00 (2013.01); C12N 15/63 (2013.01); C12N 2710/12021 (2013.01); C12N 2710/12022 (2013.01)] 8 Claims
 
1. An attenuated strain of ASFV with IPTG-induced deletion of a D1133L gene, wherein the attenuated strain of ASFV is obtained by IPTG-induced deletion of the D1133L gene of an ASFV using an Escherichia coli lac operator-repressor system; and in the absence of the IPTG, an expression of a D1133L protein of the attenuated strain of ASFV is inhibited;
wherein the Escherichia coli lac operator-repressor system comprises a vector containing SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 5;
wherein the SEQ ID NO: 5 is inserted between SEQ ID NO: 6 and SEQ ID NO: 7.