US 11,987,787 B2
Devices, methods, and kits for preparing a cell suspension
Niraj Kumar Doshi, Valencia, CA (US); Navin Noel Bunyan, Valencia, CA (US); David Allen Fencil, Valencia, CA (US); and Matthew Krywcun, Saugus, CA (US)
Assigned to AVITA Medical Americas, LLC, Valencia, CA (US)
Filed by AVITA MEDICAL AMERICAS, LLC, Valencia, CA (US)
Filed on Jul. 22, 2020, as Appl. No. 16/935,977.
Prior Publication US 2022/0025317 A1, Jan. 27, 2022
Int. Cl. C12M 1/00 (2006.01); B01L 3/00 (2006.01); B01L 9/00 (2006.01); C12M 1/33 (2006.01); C12N 5/071 (2010.01)
CPC C12M 45/22 (2013.01) [B01L 3/508 (2013.01); B01L 3/52 (2013.01); B01L 3/545 (2013.01); B01L 9/00 (2013.01); C12M 45/02 (2013.01); C12M 45/09 (2013.01); C12N 5/0625 (2013.01); B01L 2200/16 (2013.01); B01L 2200/18 (2013.01); B01L 2300/021 (2013.01); B01L 2300/025 (2013.01); B01L 2300/16 (2013.01); C12N 2509/00 (2013.01); C12N 2509/10 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A kit for preparing a cell suspension, the kit comprising:
a device defining a first reservoir, a second reservoir, and a third reservoir;
the device including:
(a) a first label identifying the first reservoir configured for use preparing the cell suspension method;
(b) a second label identifying the second reservoir configured for use preparing the cell suspension;
(c) a third label identifying the third reservoir configured for use preparing the cell suspension;
(d) a heating assembly comprising a heating mechanism configured to heat the contents of the first reservoir, wherein the heating assembly is programed to:
(i) illuminate a first visual indicator light during a first time period, the first time period comprising a period of time during which the heating mechanism heats the contents to a target temperature;
(ii) illuminate a second visual indicator light during a second time period, the second time period comprising a period of time during which the heating mechanism maintains the contents at the target temperature, wherein the second time period is at least 15 minutes; and
(iii) automatically cease operating to provide heat to the first reservoir after the second time period;
(e) one or more buttons configured to activate the heating assembly;
(f) one or more heating assembly indicators configured to be associated with indicator lights of the device to indicate one or more statuses of the device;
(g) a preparation tray configured to be used to perform the cell suspension, wherein the preparation tray is removable from a complementary recessed area of the device,
wherein the preparation tray comprises one or more collection areas disposed on a bottom surface of the preparation tray, wherein the bottom surface includes a skin tissue manipulation area, wherein the bottom surface is configured such that fluid flows from the skin tissue manipulation area along a transition surface having a slope toward the one or more collection areas, wherein the slope of the transition surface of the preparation tray is between about 0.5 degrees and about 35 degrees, and wherein the preparation tray is half-moon shaped;
(h) a housing configured to receive the device in an integrated tray portion wherein the housing is configured to function as packaging for storage and transport of the device, the housing including:
(i) a first housing portion configured to store a first set of components associated with preparing the cell suspension, wherein the first set of components includes an enzyme vial;
(ii) a the second housing portion configured to store a second set of components associated with preparing the cell suspension, wherein the first housing portion and the second housing portion are removably coupled to each other to form portions of the integrated tray portion;
(iii) a third housing portion configured to store a third set of components associated with preparing cell suspension, wherein the third housing portion is removably coupled to the first housing portion and the second housing portion; and
(iv) a base comprising a first recess configured to receive the integrated tray portion and a second recess configured to receive the third housing portion; and
(v) a box configured to receive the base.