US 11,987,778 B2
Methods of using pipe-based bioreactors for producing comestible meat products
Charles Knight Benton, Berkeley, CA (US); and Konrad Müller-Auffermann, Emeryville, CA (US)
Assigned to Upside Foods, Inc., Berkeley, CA (US)
Filed by Upside Foods, Inc., Berkeley, CA (US)
Filed on Oct. 18, 2022, as Appl. No. 18/047,545.
Application 18/047,545 is a continuation of application No. 18/047,196, filed on Oct. 17, 2022.
Prior Publication US 2024/0124817 A1, Apr. 18, 2024
Int. Cl. C12M 3/00 (2006.01); A22C 17/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 3/04 (2006.01); A23L 13/00 (2016.01)
CPC C12M 23/06 (2013.01) [A22C 17/0006 (2013.01); C12M 23/58 (2013.01); C12M 25/14 (2013.01); C12M 27/10 (2013.01); A23L 13/00 (2016.08); A23V 2002/00 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method for producing a comestible meat product, the method comprising:
flowing cells across one or more substrates disposed within an elongated bioreactor for a first period of time at an inoculation plug flow rate that displaces fluid within the elongated bioreactor in a direction in line with an inlet and an outlet of the elongated bioreactor while substantially reducing mixing of the cells with the fluid within the elongated bioreactor;
at least partially arresting flow of material through the elongated bioreactor for a second period of time to allow at least a portion of the cells to settle and adhere to the one or more substrates within the elongated bioreactor; and
circulating cell culture media from the inlet unobstructed to the one or more substrates and to the outlet of the elongated bioreactor for a third period of time at a progressively increasing flow rate to prevent shearing of the adhered cells from the one or more substrates while promoting growth of the adhered cells.