US 11,987,777 B2
High throughput, feedback-controlled electroporation microdevice for efficient molecular delivery into single cells
Jeffrey Zahn, Princeton, NJ (US); Mingde Zheng, Highland Park, NJ (US); David I. Shreiber, Whitehouse Station, NJ (US); Hao Lin, Piscataway, NJ (US); and Jerry W. Shan, Piscataway, NJ (US)
Assigned to RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY, New Brunswick, NJ (US)
Filed by Rutgers, The State University of New Jersey, New Brunswick, NJ (US)
Filed on Jan. 7, 2021, as Appl. No. 17/143,659.
Application 17/143,659 is a continuation of application No. 15/757,566, granted, now 10,927,333, previously published as PCT/US2016/050201, filed on Sep. 2, 2016.
Claims priority of provisional application 62/214,665, filed on Sep. 4, 2015.
Prior Publication US 2021/0139825 A1, May 13, 2021
Int. Cl. B01L 3/00 (2006.01); C12M 1/42 (2006.01); C12M 3/06 (2006.01); C12N 13/00 (2006.01); G01N 15/10 (2006.01); G01N 15/1031 (2024.01)
CPC C12M 1/42 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); C12M 23/16 (2013.01); C12M 35/02 (2013.01); C12N 13/00 (2013.01); G01N 15/1031 (2013.01); G01N 15/1056 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0645 (2013.01); G01N 2015/1006 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for electroporating a plurality of biological cells, the method comprising:
hydrodynamically focusing a continuous flow of a plurality of biological cells into a single-file flow, wherein the single-file flow passes each of the plurality of biological cells through a detection area;
generating a cell detection signal;
continuously monitoring an impedance value of the detection area;
generating, in response to an increase in the impedance value of the detection area over a baseline threshold, a permeabilization signal, the baseline threshold being indicative of a presence of one of the plurality of biological cells within the detection area;
stopping the permeabilization signal in response to determining that the impedance value of the detection area is equal to or greater than a permeabilization threshold value; and
generating a delivery signal, wherein the delivery signal causes delivery of a molecule into one of the plurality of biological cells focused within the detection area.