US 11,987,708 B2
Process for preparing a Lawsonia inermis extract
Laurent Subra, Montgaillard (FR); Jean-Marie Autret, Gaillac (FR); Christel Fiorini-Puybaret, Toulouse (FR); and Philippe Joulia, Villenouvelle (FR)
Assigned to PIERRE FABRE DERMO-COSMETIQUE, Boulogne-Billancourt (FR)
Appl. No. 17/608,325
Filed by PIERRE FABRE DERMO-COSMETIQUE, Boulogne-Billancourt (FR)
PCT Filed Jun. 12, 2020, PCT No. PCT/EP2020/066329
§ 371(c)(1), (2) Date Nov. 2, 2021,
PCT Pub. No. WO2020/249746, PCT Pub. Date Dec. 17, 2020.
Claims priority of application No. 1906264 (FR), filed on Jun. 12, 2019.
Prior Publication US 2022/0204776 A1, Jun. 30, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C09B 61/00 (2006.01); A61K 8/35 (2006.01); A61K 8/49 (2006.01); A61K 8/92 (2006.01); A61Q 5/06 (2006.01); C07C 46/10 (2006.01)
CPC C09B 61/00 (2013.01) [A61K 8/35 (2013.01); A61K 8/498 (2013.01); A61K 8/922 (2013.01); A61Q 5/065 (2013.01); C07C 46/10 (2013.01); A61K 2236/33 (2013.01)] 16 Claims
OG exemplary drawing
 
1. Process for preparing a lawsone-rich extract which comprises the steps of:
a) macerating the aerial parts of Lawsonia inermis in water, at a pH ranging from 4 to 8 in order for the glycosylated lawsone derivatives initially present in the aerial parts of Lawsonia inermis to be partially or totally hydrolyzed enzymatically, to provide an aqueous solution containing lawsone;
b) adding an organic solvent to the solution obtained in step a), said organic solvent being selected from C4-C12 linear, or branched, alcohols to provide an aqueous phase and an organic phase;
c) collecting the organic phase obtained from step b); and
d) concentrating the organic phase collected in step c) to obtain a lawsone-rich extract.