	US 11,987,630 B2
	Anti-vista antibodies and fragments, uses thereof, and methods of identifying same
Linda A. Snyder, Pottstown, PA (US); Gordon Powers, Malvern, PA (US); Enrique Zudaire Ubani, Ambler, PA (US); and Douglas Matthew Marvel, Lansdale, PA (US)
Assigned to Janssen Pharmaceutica NV, Beerse (BE)
Filed by Janssen Pharmaceutica NV
Filed on Nov. 25, 2020, as Appl. No. 17/104,287.
Application 17/104,287 is a division of application No. 15/430,264, filed on Feb. 10, 2017, granted, now 10,899,836.
Claims priority of provisional application 62/294,922, filed on Feb. 12, 2016.
Prior Publication US 2021/0171640 A1, Jun. 10, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); A61K 39/395 (2006.01); A61K 45/06 (2006.01); C07K 16/28 (2006.01); C07K 16/30 (2006.01); G01N 33/574 (2006.01); A61K 39/00 (2006.01)

CPC C07K 16/2827 (2013.01) [A61K 39/39558 (2013.01); A61K 45/06 (2013.01); C07K 16/3015 (2013.01); C07K 16/3023 (2013.01); C07K 16/3038 (2013.01); G01N 33/57423 (2013.01); G01N 33/6854 (2013.01); A61K 2039/505 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/33 (2013.01); C07K 2317/34 (2013.01); C07K 2317/55 (2013.01); C07K 2317/56 (2013.01); C07K 2317/565 (2013.01); C07K 2317/732 (2013.01); C07K 2317/74 (2013.01); C07K 2317/75 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01); G01N 2800/7028 (2013.01)]

2 Claims

1. A method of identifying an antibody that binds human- V-domain Ig Suppressor of T cell Activation (VISTA) protein or an antigen binding fragment thereof, from a collection of different anti-human VISTA antibodies, wherein said antibody elicits a biological response, comprising:

a) providing an antibody that binds human VISTA, or an antibody fragment thereof, and

b) determining whether the antibody or antibody fragment thereof induces a biological response in vivo in a cynomolgus monkey wherein the biological response comprises all of the following:

i) activation of monocytes;

ii) activation of T cells; and

iii) a decrease in the number of circulating immune cells.