CPC C07K 16/2827 (2013.01) [A61K 39/39558 (2013.01); A61K 45/06 (2013.01); C07K 16/3015 (2013.01); C07K 16/3023 (2013.01); C07K 16/3038 (2013.01); G01N 33/57423 (2013.01); G01N 33/6854 (2013.01); A61K 2039/505 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/33 (2013.01); C07K 2317/34 (2013.01); C07K 2317/55 (2013.01); C07K 2317/56 (2013.01); C07K 2317/565 (2013.01); C07K 2317/732 (2013.01); C07K 2317/74 (2013.01); C07K 2317/75 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01); G01N 2800/7028 (2013.01)] | 2 Claims |
1. A method of identifying an antibody that binds human- V-domain Ig Suppressor of T cell Activation (VISTA) protein or an antigen binding fragment thereof, from a collection of different anti-human VISTA antibodies, wherein said antibody elicits a biological response, comprising:
a) providing an antibody that binds human VISTA, or an antibody fragment thereof, and
b) determining whether the antibody or antibody fragment thereof induces a biological response in vivo in a cynomolgus monkey wherein the biological response comprises all of the following:
i) activation of monocytes;
ii) activation of T cells; and
iii) a decrease in the number of circulating immune cells.
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