US 11,987,619 B2
Single-chain chimeric polypeptides and uses thereof
Hing Wong, Miramar, FL (US)
Assigned to HCW Biologics, Inc., Miramar, FL (US)
Filed by HCW Biologics, Inc., Miramar, FL (US)
Filed on Jul. 21, 2022, as Appl. No. 17/870,373.
Application 17/870,373 is a division of application No. 16/556,040, filed on Aug. 29, 2019, granted, now 11,401,324.
Claims priority of provisional application 62/881,039, filed on Jul. 31, 2019.
Claims priority of provisional application 62/817,241, filed on Mar. 12, 2019.
Claims priority of provisional application 62/817,244, filed on Mar. 12, 2019.
Claims priority of provisional application 62/816,683, filed on Mar. 11, 2019.
Claims priority of provisional application 62/749,506, filed on Oct. 23, 2018.
Claims priority of provisional application 62/746,832, filed on Oct. 17, 2018.
Claims priority of provisional application 62/725,038, filed on Aug. 30, 2018.
Prior Publication US 2023/0039157 A1, Feb. 9, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/18 (2006.01); A61P 3/10 (2006.01); C07K 16/28 (2006.01); C12N 5/0783 (2010.01); A61K 39/00 (2006.01)
CPC C07K 16/18 (2013.01) [A61P 3/10 (2018.01); C07K 16/2809 (2013.01); C07K 16/2818 (2013.01); C07K 16/2866 (2013.01); C12N 5/0637 (2013.01); A61K 39/00 (2013.01); C07K 2317/24 (2013.01); C07K 2317/56 (2013.01); C07K 2317/622 (2013.01); C07K 2319/00 (2013.01); C12N 2501/998 (2013.01)] 27 Claims
 
1. A nucleic acid encoding a single-chain chimeric polypeptide comprising:
(i) a first target-binding domain comprising a sequence at least 80% identical to SEQ ID NO: 28;
(ii) a soluble tissue factor domain that does not have coagulation activity, wherein the soluble tissue factor domain comprises a sequence at least 80% identical to SEQ ID NO: 9; and
(iii) a second target-binding domain comprising a sequence at least 80% identical to SEQ ID NO: 28,
wherein the single-chain chimeric polypeptide does not stimulate blood coagulation in a mammal.