US 11,986,825 B1
Multi-step kinetic immunoassay systems and methods
Yann Astier, Oakland, CA (US); and Juraj Topolancik, Redwood City, CA (US)
Assigned to Panazee Inc., Oakland, CA (US)
Filed by Panazee Inc., Oakland, CA (US)
Filed on May 5, 2023, as Appl. No. 18/313,183.
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/00 (2006.01); G01N 33/533 (2006.01)
CPC B01L 3/502761 (2013.01) [G01N 33/533 (2013.01); B01L 2300/0636 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method for analyzing reaction kinetics of a binding reaction, the method comprising:
mixing a first plurality of beads with a sample to form a first mixture in a reactor of a microfluidic chip, wherein:
the sample comprises a plurality of analytes,
each bead of the first plurality of beads is coupled to an affinity reagent, and
the affinity reagent is configured to bind to the analyte;
binding a first subset of the plurality of analytes to a plurality of affinity reagents coupled to the first plurality of beads in the reactor;
flowing a first portion of the first mixture from the reactor through a first fluidic path defined by a plurality of structures in the microfluidic chip, wherein:
the first portion of the first mixture comprises the first plurality of beads coupled to a first subset of the plurality of affinity reagents,
the plurality of structures comprises a plurality of pillars,
the plurality of pillars is a portion of an array of pillars, and
the array of pillars is characterized by a plurality of rows and a plurality of columns;
coupling the first plurality of beads to a plurality of label compounds in the first fluidic path;
flowing a solution in a second fluidic path, wherein the second fluidic path intersects the first fluidic path; and
measuring an amount of the plurality of label compounds coupled to the first plurality of beads.