US 11,986,823 B2
Microfluidic device and method for the nanostructure sequencing of nucleotide strands
Franz Laermer, Weil der Stadt (DE)
Assigned to Robert Bosch GmbH, Stuttgart (DE)
Appl. No. 17/053,012
Filed by Robert Bosch GmbH, Stuttgart (DE)
PCT Filed May 2, 2019, PCT No. PCT/EP2019/061247
§ 371(c)(1), (2) Date Jan. 19, 2021,
PCT Pub. No. WO2019/215005, PCT Pub. Date Nov. 14, 2019.
Claims priority of application No. 10 2018 207 098.9 (DE), filed on May 8, 2018.
Prior Publication US 2021/0138465 A1, May 13, 2021
Int. Cl. B01L 1/00 (2006.01); B01L 3/00 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01)
CPC B01L 3/502761 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01); B01L 2200/0663 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0896 (2013.01); C12Q 2531/113 (2013.01); G01N 2223/07 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A microfluidic device for sequencing nucleotide strands, the microfluidic device comprising:
an array unit comprising array cells having substances for an asymmetric polymerase chain reaction, the array cells including nucleotides having stop properties according to the Sanger sequencing method and primers for the asymmetric polymerase chain reaction; and
a nanostructure connected to the array unit and configured to determine lengths of nucleotide strands formed by the polymerase chain reaction.