US 11,986,521 B2
Viral particle-based vaccine
Bodo Plachter, Ansbach (DE); and Inessa Penner, Mainz-Kastel (DE)
Assigned to VAKZINE PROJEKT MANAGEMENT GMBH, Hannover (DE)
Appl. No. 15/734,634
Filed by VAKZINE PROJEKT MANAGEMENT GMBH, Hannover (DE)
PCT Filed Jun. 7, 2019, PCT No. PCT/EP2019/064941
§ 371(c)(1), (2) Date Dec. 3, 2020,
PCT Pub. No. WO2019/234219, PCT Pub. Date Dec. 12, 2019.
Claims priority of application No. 18176735 (EP), filed on Jun. 8, 2018.
Prior Publication US 2021/0228711 A1, Jul. 29, 2021
Int. Cl. A61K 39/245 (2006.01); A61K 39/00 (2006.01); A61P 31/22 (2006.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01)
CPC A61K 39/245 (2013.01) [A61P 31/22 (2018.01); C07K 14/005 (2013.01); C12N 7/00 (2013.01); A61K 2039/5252 (2013.01); A61K 2039/545 (2013.01); C12N 2710/16121 (2013.01); C12N 2710/16122 (2013.01); C12N 2710/16134 (2013.01)] 18 Claims
 
1. A nucleic acid molecule encoding the genome of a recombinant human cytomegalovirus (HCMV) Towne strain, wherein the Towne strain sequence of SEQ ID NO: 6 is modified to comprise the elements necessary to express the genome as a Bacterial Artificial Chromosome (BAC), wherein the recombinant HCMV comprises an open reading frame (ORF) encoding Green Fluorescent Protein (GFP) which is disrupted by the insertion of a gene heterologous to HCMV Towne strain, wherein the recombinant HCMV encodes a functional heterologous UL130 protein, and wherein the nucleic acid molecule has an identity of at least 90% when compared to the genome of the HCMV strain Towne as deposited under GenBank accession no: AY315197 (SEQ ID NO: 6).