US 11,986,288 B2
Colorometric sensor for the non-invasive screening of glucose in sweat in pre and type 2 diabetes
Ashwin K. Rao, West Hills, CA (US); Rebecca K. Gottlieb, Culver City, CA (US); and Quyen Ong, Arcadia, CA (US)
Assigned to MEDTRONIC MINIMED, INC., Northridge, CA (US)
Filed by MEDTRONIC MINIMED, INC., Northridge, CA (US)
Filed on Mar. 5, 2018, as Appl. No. 15/912,473.
Claims priority of provisional application 62/467,653, filed on Mar. 6, 2017.
Prior Publication US 2018/0303388 A1, Oct. 25, 2018
This patent is subject to a terminal disclaimer.
Int. Cl. A61B 5/145 (2006.01); A61B 5/1477 (2006.01); A61B 5/1486 (2006.01); G01N 33/52 (2006.01); G01N 33/66 (2006.01); A61B 5/00 (2006.01); C07K 14/62 (2006.01); C12Q 1/00 (2006.01)
CPC A61B 5/14532 (2013.01) [A61B 5/14517 (2013.01); A61B 5/1486 (2013.01); G01N 33/526 (2013.01); G01N 33/66 (2013.01); A61B 5/14539 (2013.01); A61B 5/14546 (2013.01); A61B 5/1477 (2013.01); A61B 5/6833 (2013.01); C07K 14/62 (2013.01); C12Q 1/006 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A composition of matter comprising:
a glucose sensing enzyme complex that reacts with glucose and comprises:
glucose oxidase, glucose dehydrogenase or a hexokinase/glucose-6-phosphate complex;
a colorimetric indicator that changes color following reaction of glucose with the glucose sensing enzyme complex; and
a predefined concentration of glucose adapted to form a threshold concentration of glucose in an aqueous environment as the glucose sensing enzyme complex senses glucose; wherein:
the composition is disposed within a porous substrate;
the porous substrate comprises at least one channel in operable contact with the glucose sensing enzyme complex;
the porous substrate is coupled to a layer of an adhesive adapted to adhere to human skin;
the layer of the adhesive comprises an opening adapted to allow sweat to flow from human skin into the porous substrate, wherein the opening is operably connected to the at least one channel; and
the opening, the at least one channel and the glucose sensing complex are disposed in the composition such that concentrations of glucose can be sensed in sweat that flows from human skin into the opening and through the at least one channel to the glucose sensing enzyme complex.