US 11,986,166 B2
Real-time diagnosis of cancer involved sentinel lymph nodes (SLNS) based on pH sensing
Mohammad Abdolahad, Tehran (IR); Zohreh Sadat Miripour, Tehran (IR); and Parisa Aghaee, Tehran (IR)
Assigned to NANO HESGARSAZAN SALAMAT ARYA, (IR)
Filed by Mohammad Abdolahad, Tehran (IR); Zohreh Sadat Miripour, Tehran (IR); and Parisa Aghaee, Tehran (IR)
Filed on Mar. 9, 2021, as Appl. No. 17/195,680.
Claims priority of provisional application 62/987,884, filed on Mar. 11, 2020.
Prior Publication US 2021/0223246 A1, Jul. 22, 2021
Int. Cl. A61B 5/1473 (2006.01); A61B 5/00 (2006.01); A61B 5/145 (2006.01); A61B 10/00 (2006.01)
CPC A61B 10/0041 (2013.01) [A61B 5/14539 (2013.01); A61B 5/1473 (2013.01); A61B 5/418 (2013.01); A61M 2202/0405 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A method for detecting cancer involved lymph nodes, comprising:
in vivo measuring pH value of lymphatic fluid of a lymph node, comprising:
placing a pH-sensing paper inside a hollow needle of an injection syringe by adhering the pH-sensing paper inside the hollow needle, one end of the pH-sensing paper at a tip of the hollow needle being in contact with a surrounding environment of the hollow needle;
filling the injection syringe with a buffer solution;
putting the pH-sensing paper in in-situ contact with lymphatic fluid of the lymph node inside the lymph node by putting the one end of the pH-sensing paper inside the lymph node via inserting the tip of the hollow needle of the injection syringe inside the lymph node; and
putting the lymphatic fluid in interaction with the buffer solution by injecting the buffer solution through the hollow needle of the injection syringe into the lymph node utilizing the injection syringe, the interacted lymphatic fluid of the lymph node with the buffer solution being in contact with the one end of the pH-sensing paper; and
detecting the lymph node being a cancer involved lymph node responsive to the measured pH value of the lymphatic fluid of the lymph node being less than 7.0 by detecting changing color of the pH-sensing paper to an acidic-range pH color.