US 11,985,932 B2
Unnatural plant showing induced gene expression and method for producing same
Issei Kobayashi, Mie (JP)
Assigned to National University Corporation Mie University, Tsu (JP)
Appl. No. 17/605,861
Filed by National University Corporation Mie University, Mie (JP)
PCT Filed Apr. 21, 2020, PCT No. PCT/JP2020/017159
§ 371(c)(1), (2) Date Oct. 26, 2021,
PCT Pub. No. WO2020/218279, PCT Pub. Date Oct. 29, 2020.
Claims priority of application No. 2019-083683 (JP), filed on Apr. 25, 2019.
Prior Publication US 2022/0210990 A1, Jul. 7, 2022
Int. Cl. A01H 3/04 (2006.01); A01H 6/46 (2018.01); A01H 6/82 (2018.01)
CPC A01H 3/04 (2013.01) [A01H 6/4636 (2018.05); A01H 6/823 (2018.05)] 25 Claims
 
1. A method for producing a plant in which expression of a gene is induced, comprising the steps of:
(1) providing a portion of a plant;
(2) culturing the portion of the plant under dedifferentiation-inducing conditions to form a callus;
(3) culturing the callus under redifferentiation-inducing conditions to form a shoot;
(A) providing a stimulus to the callus, wherein the stimulus is a stimulus not reducing the survival rate of the callus to 90% or less, as compared to a control to which the stimulus is not provided; and
(4) culturing the shoot to obtain the plant in which the expression of the gene is induced in the absence of the stimulus, wherein
steps (1), (2), (3), and (4) are performed in the order of (1), (2), (3), and (4), and step (A) is performed in either or both of steps (2) and (3), wherein
in step (2) or (3), the callus is not selected by using a resistance to the stimulus as an index, and wherein
the stimulus is a stimulus inducing the expression of the gene in the callus.