US 11,657,893 B2
Models for analyzing data from sequencing-by-synthesis operations
Todd Rearick, Cheshire, CT (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on May 11, 2020, as Appl. No. 15/929,565.
Application 15/498,591 is a division of application No. 13/892,116, filed on May 10, 2013, granted, now 9,646,132, issued on May 9, 2017.
Application 15/929,565 is a continuation of application No. 15/498,591, filed on Apr. 27, 2017, granted, now 10,679,724.
Claims priority of provisional application 61/645,951, filed on May 11, 2012.
Prior Publication US 2020/0342952 A1, Oct. 29, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/48 (2006.01); G01N 33/50 (2006.01); G16B 5/00 (2019.01); C12Q 1/6874 (2018.01); G16B 25/00 (2019.01); G16B 30/00 (2019.01)
CPC G16B 5/00 (2019.02) [C12Q 1/6874 (2013.01); G16B 25/00 (2019.02); G16B 30/00 (2019.02)] 18 Claims
OG exemplary drawing
 
1. A system for sequencing-by-synthesis, comprising:
a reactor array having multiple reaction confinement regions, one or more copies of a polynucleotide strand being located in a loaded reaction confinement region of the reactor array, the loaded reaction confinement region being located in a vicinity of one or more neighboring reaction confinement regions;
a machine-readable memory; and
a processor communicatively coupled to the reactor array and the memory, the processor configured to execute machine-readable instructions, which, when executed by the processor, cause the system to perform a method, comprising:
receiving output signals at the processor from the reactor array in response to flowing a series of nucleotide flows onto the reactor array, and
estimating a background signal for the loaded reaction confinement region using the received output signals and a background model representing at least an exchange of ions between the one or more neighboring reaction confinement regions and a headspace adjacent to the loaded reaction confinement region and the one or more neighboring reaction confinement regions, wherein the background model is derived using a first characteristic equation, wherein the first characteristic equation comprises a term related to a difference between a proton concentration in each of the neighboring reaction confinement regions and a proton concentration in the headspace.