US 11,656,448 B2
Method and apparatus for quantitative hyperspectral fluorescence and reflectance imaging for surgical guidance
Pablo A. Valdes, Hanover, NH (US); David W. Roberts, Lyme, NH (US); Keith D. Paulsen, Hanover, NH (US); and Frederic Leblond, Montreal (CA)
Assigned to THE TRUSTEES OF DARTMOUTH COLLEGE, Hanover, NH (US)
Filed by THE TRUSTEES OF DARTMOUTH COLLEGE, Hanover, NH (US)
Filed on Jun. 17, 2019, as Appl. No. 16/443,760.
Application 16/443,760 is a continuation of application No. 14/373,443, abandoned, previously published as PCT/US2013/022266, filed on Jan. 18, 2013.
Claims priority of provisional application 61/588,708, filed on Jan. 20, 2012.
Prior Publication US 2019/0384048 A1, Dec. 19, 2019
Int. Cl. G02B 21/06 (2006.01); G02B 21/36 (2006.01); A61B 1/06 (2006.01)
CPC G02B 21/06 (2013.01) [A61B 1/063 (2013.01); G02B 21/36 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A hyperspectral imaging method for determining depth and concentration of fluorophores in tissue of a patient, comprising:
capturing a white-light hyperspectral image cube of the tissue as illuminated with white light, the capturing performed using a hyperspectral imaging device that passes light from the tissue through a tunable filter into a broad-spectrum electronic camera;
capturing, using the hyperspectral imaging device, a fluorescence-light hyperspectral image cube of the tissue as illuminated with fluorescence stimulus light;
analyzing spectral bands of the white-light hyperspectral image cube near a wavelength of the fluorescence stimulus light and a wavelength of associated fluorescence emissions to extract scattering and absorption parameters of the tissue at the wavelengths of the fluorescence stimulus light and the associated fluorescence emissions;
normalizing at least one selected pair of images of the fluorescence-light hyperspectral image cube, the selected pair of images being associated with two different respective wavelengths near a peak wavelength of the fluorescence emission;
deriving, based upon the scattering and absorption parameters, the depth of fluorophores in the tissue at pixels from a ratio between the two images of the at least one selected pair of images of the fluorescence-light hyperspectral image cube, as normalized; and
determining, using a voxel-based model and the extracted scattering and absorption parameters derived from the white-light hyperspectral image cube, and the depth of the fluorophores in the tissue at the pixels and at least one image of the fluorescence-light hyperspectral image cube, fluorophore concentration at voxels of the voxel-based model; wherein
the step of deriving comprises comparing the ratio between the two images of the at least one selected pair of images to a model of depth-dependent spectral properties of the fluorescence of the two images of the at least one selected pair of images.