US 11,656,235 B2
DNA aptamer-cyanine complexes as mephedrone and cannabinoid colorimetric sensors
Yi Xiao, Miami, FL (US); Obtin Alkhamis, Miami, FL (US); and Haixiang Yu, Miami, FL (US)
Assigned to THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES, Miami, FL (US)
Filed by Yi Xiao, Miami, FL (US); Obtin Alkhamis, Miami, FL (US); and Haixiang Yu, Miami, FL (US)
Filed on Aug. 7, 2021, as Appl. No. 17/396,663.
Prior Publication US 2023/0054132 A1, Feb. 23, 2023
Int. Cl. G01N 33/94 (2006.01); G01N 21/78 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/9486 (2013.01) [G01N 21/78 (2013.01); G01N 33/583 (2013.01); G01N 33/946 (2013.01); G01N 33/948 (2013.01); G01N 33/9413 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for detecting mephedrone in a sample, the method comprising
a) mixing an aptamer with a dye to form an aptamer-dye complex, the aptamer comprising a nucleic acid sequence selected from SEQ ID NO: 7 and sequences sharing at least 95% sequence identity with SEQ ID NO: 7, and the dye being MTC;
b) mixing/contacting the aptamer-dye complex with the sample; and
c) detecting mephedrone in the sample by detecting an optical change generated upon the assembly of an aptamer-mephedrone complex and the disassembly of the aptamer-dye complex.
 
9. A method for detecting mephedrone in a sample, the method comprising
mixing/contacting non-covalent assemblies of aptamer-dye complex with the sample, the dye being MTC, and the aptamer comprising a nucleic acid sequence selected from SEQ ID NO: 7 and sequences sharing at least 95% sequence identity with SEQ ID NO: 7;
measuring absorbance at 585-590 nm and 650-655 nm; and
detecting methedrone in the sample by detecting a change in absorbance measured at 585-590 nm and 650-655 nm.
 
15. A method for detecting a cannabinoid in a sample, the method comprising
a) mixing an aptamer with a dye to form an aptamer-dye complex, the aptamer comprising a nucleic acid sequence selected from SEQ ID NO: 4 and sequences sharing at least 95% sequence identity with SEQ ID NO: 4, and the dye being MTC;
b) mixing/contacting the aptamer-dye complex with the sample; and
c) detecting the cannabinoid in the sample by detecting an optical change generated upon the assembly of an aptamer-cannabinoid complex and the disassembly of the aptamer-dye complex.