US 11,656,223 B2
Method of detecting test substance, sample analysis cartridge, and sample analyzer
Kazuyoshi Horii, Kobe (JP); Tomoyuki Nose, Kobe (JP); Takao Fujiwara, Kobe (JP); and Tatsuya Kosako, Kobe (JP)
Assigned to SYSMEX CORPORATION, Kobe (JP)
Filed by SYSMEX CORPORATION, Kobe (JP)
Filed on Jun. 24, 2021, as Appl. No. 17/356,597.
Application 17/356,597 is a continuation of application No. 16/575,449, filed on Sep. 19, 2019, granted, now 11,073,514.
Application 16/575,449 is a continuation of application No. 15/081,070, filed on Mar. 25, 2016, granted, now 10,473,652, issued on Nov. 12, 2019.
Claims priority of application No. 2015-093387 (JP), filed on Apr. 30, 2015.
Prior Publication US 2021/0318297 A1, Oct. 14, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/00 (2006.01); G01N 33/543 (2006.01); G01N 33/48 (2006.01); B01F 25/433 (2022.01); B01F 31/65 (2022.01); B01F 31/441 (2022.01); B01F 33/452 (2022.01)
CPC G01N 33/54326 (2013.01) [B01F 25/4331 (2022.01); B01F 25/4338 (2022.01); B01F 31/441 (2022.01); B01F 31/65 (2022.01); B01F 33/452 (2022.01); B01L 3/50273 (2013.01); B01L 3/502761 (2013.01); G01N 33/54306 (2013.01); G01N 33/54366 (2013.01); B01L 3/502715 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0874 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/043 (2013.01); G01N 33/48 (2013.01)] 13 Claims
OG exemplary drawing
 
9. A cartridge-based sample analysis method, comprising:
forming, in a liquid phase in a chamber of a cartridge, a complex including an analyte in a sample, a magnetic particle and a labeled substance, the magnetic particle and the labeled substance being bound to the analyte;
attracting the complex in the liquid phase in the chamber with a magnetic source;
transferring the complex, by moving the magnetic source relatively to the cartridge while attracting the magnetic particle with the moving magnetic source moving along an external surface of the cartridge, from the liquid phase in the chamber to a passage which is connected to the chamber and where a gas phase is defined in the cartridge;
transferring the complex, by moving the magnetic source relatively to the cartridge while attracting the magnetic particle with the moving magnetic source, from the gas phase to a detection tank where the labeled substance in the complex is reacted with a substrate to generate a detectable signal; and
detecting the sample based on the detectable signal in the detection tank.