US 11,656,167 B2
Droplet sorting based on ionization mass spectrometry
Clive Adrian Smith, Hertford (GB); Xin Li, Cambridge (GB); and Frank F. Craig, Cambridge (GB)
Assigned to Sphere Fluidics Limited, Cambridge (GB)
Filed by Sphere Fluidics Limited, Cambridge (GB)
Filed on Dec. 16, 2019, as Appl. No. 16/716,369.
Application 16/716,369 is a continuation of application No. 15/502,630, granted, now 10,545,083, previously published as PCT/GB2015/052301, filed on Aug. 7, 2015.
Claims priority of application No. 1414177 (GB), filed on Aug. 11, 2014.
Prior Publication US 2020/0116621 A1, Apr. 16, 2020
Int. Cl. G01N 15/10 (2006.01); B01L 3/00 (2006.01); G01N 15/14 (2006.01); H01J 49/00 (2006.01); H01J 49/04 (2006.01)
CPC G01N 15/1031 (2013.01) [B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502784 (2013.01); G01N 15/1056 (2013.01); G01N 15/1404 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2400/0424 (2013.01); B01L 2400/0427 (2013.01); B01L 2400/084 (2013.01); G01N 2015/1081 (2013.01); G01N 2015/149 (2013.01); G01N 2015/1486 (2013.01); H01J 49/0031 (2013.01); H01J 49/04 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for aligning sequences of droplets in streams, the method comprising:
providing an emulsion comprising a plurality of target droplets and a plurality of tag droplets, wherein a tag droplet comprises a first tag and a second tag;
splitting the target droplet into a first target droplet and a second target droplet and splitting the tag droplet into a first tag droplet and a second tag droplet, wherein both the first tag droplet and the second tag droplet comprise the first tag and the second tag;
providing the first target droplet and the first tag droplet into a first stream of droplets, and providing the second target droplet and the second tag droplet into a second stream of droplets;
detecting the first tag droplets and the first target droplets in the first stream and detecting the second tag droplets and the second target droplets in the second stream;
determining a first sequence of droplets in the first stream based on the detection of the first tag droplets and the first target droplets, and determining a second sequence of droplets in the second stream based on the detection of the second tag droplets and the second target droplets; and
comparing the determined first and second sequences to align the first and second sequences.