US 11,655,504 B2
High intensity labeled reactant compositions and methods for sequencing
Jonathan M. Rothberg, Guilford, CT (US); Jeremy Lackey, Guilford, CT (US); Brian Reed, Madison, CT (US); Xinghua Shi, Madison, CT (US); Haidong Huang, Madison, CT (US); and David Dodd, Guilford, CT (US)
Assigned to Quantum-Si Incorporated, Guilford, CT (US)
Filed by Quantum-Si Incorporated, Guilford, CT (US)
Filed on Jul. 24, 2018, as Appl. No. 16/43,547.
Claims priority of provisional application 62/536,426, filed on Jul. 24, 2017.
Prior Publication US 2019/0024168 A1, Jan. 24, 2019
Int. Cl. C12Q 1/6876 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6806 (2018.01); C07H 19/073 (2006.01); C07H 21/04 (2006.01); C07H 19/173 (2006.01); C12Q 1/6869 (2018.01)
CPC C12Q 1/6876 (2013.01) [C07H 19/073 (2013.01); C07H 19/173 (2013.01); C07H 21/04 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6869 (2013.01); C12Q 2525/185 (2013.01); C12Q 2525/197 (2013.01); C12Q 2525/313 (2013.01); C12Q 2563/103 (2013.01); C12Q 2600/16 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A labeled molecule comprising a reactant connected to two or more luminescent labels via a nucleic acid linker, wherein each luminescent label is at least 5 angstroms separated from any other luminescent label, wherein
(i) at least one luminescent label is integrated within the nucleic acid linker, and wherein the at least one luminescent label that is integrated within the nucleic acid linker is a cyanine-based fluorophore; and/or
(ii) wherein at least one luminescent label is attached to the nucleic acid linker via a glycolamine spacer molecule, and wherein the labeled nucleotide comprises the structure:

OG Complex Work Unit Chemistry
where custom character is the at least one luminescent label that is attached to the nucleic acid linker via the glycolamine spacer molecule.