US 11,655,501 B2
Methods for processing paired end sequences
Gilad Almogy, Palo Alto, CA (US); Mark Pratt, Bozeman, MT (US); and Florian Oberstrass, Menlo Park, CA (US)
Assigned to ULTIMA GENOMICS INC., Newark, CA (US)
Filed by ULTIMA GENOMICS, INC., Newark, CA (US)
Filed on Apr. 7, 2020, as Appl. No. 16/842,584.
Application 16/842,584 is a continuation of application No. PCT/US2018/056593, filed on Oct. 18, 2018.
Claims priority of provisional application 62/574,629, filed on Oct. 19, 2017.
Prior Publication US 2020/0308640 A1, Oct. 1, 2020
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6869 (2013.01) [C12Q 1/6806 (2013.01)] 30 Claims
 
1. A method for processing a nucleic acid molecule, comprising:
(a) providing a partition of a plurality of partitions, wherein said partition comprises (i) said nucleic acid molecule, wherein said nucleic acid molecule comprises a first strand and a second strand, wherein said first strand has sequence complementarity with said second strand, wherein said first strand is coupled to a first adaptor, and wherein said second strand is coupled to a second adaptor; (ii) a first set of beads, wherein a first bead of said first set of beads comprises a first primer having sequence complementarity with said first adaptor coupled to said first strand of said nucleic acid molecule; and (iii) a second set of beads, wherein a second bead of said second set of beads comprises a second primer having sequence complementarity with said second adaptor coupled to said second strand of said nucleic acid molecule, wherein said first primer and said second primer comprise different nucleic acid sequences; and
(b) subjecting said partition to conditions sufficient to generate (i) one or more copies of said first strand, or a complement thereof, using said first primer coupled to said first bead and (ii) one or more copies of said second strand, or a complement thereof, using said second primer coupled to said second bead, wherein said one or more copies of said first strand, or said complement thereof, at least partially overlap said one or more copies of said second strand, or said complement thereof.