US 11,655,462 B2
Mutant microorganism introduced with highly active malate dehydrogenase for producing succinic acid and method of producing succinic acid using the same
Sang Yup Lee, Daejeon (KR); Kyung-Jin Kim, Daejeon (KR); Jung Ho Ahn, Daejeon (KR); Hogyun Seo, Daejeon (KR); and Jong An Lee, Daejeon (KR)
Assigned to KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, Daejeon (KR)
Appl. No. 16/490,515
Filed by KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, Daejeon (KR)
PCT Filed May 2, 2019, PCT No. PCT/KR2019/005247
§ 371(c)(1), (2) Date Aug. 30, 2019,
PCT Pub. No. WO2020/075943, PCT Pub. Date Apr. 16, 2020.
Claims priority of application No. 10-2018-0120696 (KR), filed on Oct. 10, 2018.
Prior Publication US 2021/0332332 A1, Oct. 28, 2021
Int. Cl. C12N 1/20 (2006.01); C12N 9/04 (2006.01); C12N 15/74 (2006.01); C12P 7/46 (2006.01)
CPC C12N 9/0006 (2013.01) [C12N 1/20 (2013.01); C12N 15/74 (2013.01); C12P 7/46 (2013.01); C12Y 101/01037 (2013.01)] 4 Claims
 
1. A mutant microorganism in which the gene encoding a malate dehydrogenase is introduced into Mannheimia succiniciproducens PALK (KCTC10973BP),
wherein the malate dehydrogenase comprises the amino acid sequence of SEQ ID NO: 40 or the amino acid sequence of SEQ ID NO: 44, and a gene encoding malate dehydrogenase present in the genome of Mannheimia succiniciproducens PALK (KCTC10973BP) is deleted.