US 11,655,442 B2
Cell bio-incubator with a variable internal pressure
Arno Gutleb, Remich (LU); Tommaso Serchi, Kirsch-les-Sierck (FR); and Mathieu Gerard, Rehon (FR)
Assigned to LUXEMBOURG INSTITUTE OF SCIENCE AND TECHNOLOGY (LIST), Luxembourg (LU)
Appl. No. 16/954,235
Filed by LUXEMBOURG INSTITUTE OF SCIENCE AND TECHNOLOGY (LIST), Esch-sur-Alzette (LU)
PCT Filed Dec. 31, 2018, PCT No. PCT/EP2018/086900
§ 371(c)(1), (2) Date Jun. 16, 2020,
PCT Pub. No. WO2019/129799, PCT Pub. Date Jul. 4, 2019.
Claims priority of application No. LU100595 (LU), filed on Dec. 27, 2017.
Prior Publication US 2020/0339937 A1, Oct. 29, 2020
Int. Cl. C12M 1/42 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01)
CPC C12M 35/04 (2013.01) [C12M 29/14 (2013.01); C12M 41/14 (2013.01); C12M 41/40 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A system for incubating one or more cells and organotypic cultures for biological investigation and toxicology assessment, said system comprising:
a bio-incubator with at least one orifice;
a pressure system fluidly connected with the bio-incubator through the at least one orifice;
wherein the bio-incubator and the gas pressure system form a hermetically closed circuit;
wherein the pressure system is a cyclic gas pressure system configured for cyclically varying the gas pressure in the bio-incubator between a negative pressure of at least −1 mbar and a positive pressure of at least +1 mbar compared to the atmospheric pressure, so as to reproduce the pressure conditions in lungs of a living mammal; and the orifice comprises a feedthrough with a pipe comprising inside the bio-incubator a portion curved towards a bottom part or a top part of the bio-incubator configured to deviate the air influx from the one or more cells and/or organotypic cultures, the curved portion terminating with a silencer for decreasing the air influx within the bio-incubator.