US 11,655,270 B2
Method for separating collagen from liposuction effluent using supercritical process
YongWoo Shin, Seongnam-si (KR); Hyoung Soon Kim, Incheon (KR); Yoonkook Park, Sejong-si (KR); Yong-Soo Choi, Gunpo-si (KR); Kyu-byung Kim, Gwangju-si (KR); Seong Sik You, Pyeongtaek-si (KR); Gapsu Han, Seoul (KR); and Seong-Rae No, Gwangju (KR)
Assigned to DOF INC., Seongnam-Si (KR)
Appl. No. 16/322,894
Filed by DOF INC., Seongnam-si (KR)
PCT Filed Aug. 8, 2017, PCT No. PCT/KR2017/008543
§ 371(c)(1), (2) Date Feb. 1, 2019,
PCT Pub. No. WO2018/030748, PCT Pub. Date Feb. 15, 2018.
Claims priority of application No. 10-2016-0100641 (KR), filed on Aug. 8, 2016; and application No. 10-2017-0100339 (KR), filed on Aug. 8, 2017.
Prior Publication US 2021/0332081 A1, Oct. 28, 2021
Int. Cl. C07K 1/14 (2006.01); B01D 11/02 (2006.01); C07K 14/78 (2006.01)
CPC C07K 1/14 (2013.01) [B01D 11/0203 (2013.01); C07K 14/78 (2013.01)] 1 Claim
1. A method for extracting Type I collagen in a polymer state from a liposuction effluent using supercritical fluid extraction comprising,
(a) introducing a liposuction effluent into a supercritical extraction device and supplying CO2, which is an extraction solvent, to an extraction reactor, and further adding ethanol as a co-solvent in an amount of 50 to 200% (w/w) with respect to the liposuction effluent;
(b) maintaining the pressure of the CO2 supplied to the extraction reactor at 200 to 300 bar and the temperature at 30 to 35° C. to extract lipids through an outlet provided with a cooler and remove the extracted lipids; and
(c) adjusting the pressure to atmospheric pressure and separating the reactor to obtain the Type I collagen in the polymer state,
wherein the molecular weight of the Type I collagen in the polymer state is 140 kDa or greater and 300 kDa or less.