US 11,654,434 B2
Microinjection chip, device, system and uses thereof for unicellular or multicellular organisms
Shawn Lockery, Eugene, OR (US)
Assigned to NemaMetrix Inc., Eugene, OR (US); and University of Oregon, Eugene, OR (US)
Filed by NemaMetrix Inc., Eugene, OR (US); and University of Oregon, Eugene, OR (US)
Filed on Jun. 24, 2019, as Appl. No. 16/449,707.
Claims priority of provisional application 62/689,245, filed on Jun. 24, 2018.
Prior Publication US 2019/0388892 A1, Dec. 26, 2019
Int. Cl. B01L 3/00 (2006.01); C12M 1/26 (2006.01); A01K 67/033 (2006.01); C12N 15/89 (2006.01)
CPC B01L 3/502761 (2013.01) [A01K 67/0336 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502753 (2013.01); C12M 33/04 (2013.01); C12N 15/89 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A microinjection device for injection into unicellular or multicellular organisms, comprising a microinjection pipette, a microinjection chip and a backing bonded to the microinjection chip wherein the microinjection chip is molded and monolithic comprising, in fluid communication with each other:
a) an inlet port comprising an exterior side wall having a first width;
b) a preinjection reservoir;
c) an injection channel that does not comprise a microinjection port and comprises an exterior sidewall having a second width less than the first width, wherein the exterior side wall of the injection channel has a thickness from 10 μm to 40 μm and wherein the microinjection pipette is positioned adjacent to the exterior side wall for injection; and,
d) a post injection reservoir comprising an exterior side wall having a third width greater than the second width;
wherein:
the backing forms a bottom to the inlet port, the injection channel and the post injection reservoir;
the inlet port is adapted to sequentially move an individual organism into the injection channel, which is adapted by size to immobilize the individual organism in fluid, and,
the exterior side wall of the injection channel is configured to: i) receive the microinjection pipette without obstruction from any component of the microinjection chip other than the sidewall; ii) be directly penetrable by the microinjection pipette, without a microinjection port; and, iii) reseal upon removal of the microinjection pipette.