US 11,654,431 B2
Systems, devices and methods for microfluidic analysis
Robert Justice Shartle, Livermore, CA (US); Sherb M. Edmondson, Jr., Sequim, WA (US); and Bob Larson, Fremont, CA (US)
Assigned to Zoetis Services LLC, Parsippany, NJ (US)
Filed by ZOETIS SERVICES LLC, Parsippany, NJ (US)
Filed on Oct. 3, 2019, as Appl. No. 16/591,814.
Application 16/591,814 is a continuation of application No. PCT/US2018/028855, filed on Apr. 23, 2018.
Claims priority of provisional application 62/488,377, filed on Apr. 21, 2017.
Prior Publication US 2020/0030797 A1, Jan. 30, 2020
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 2300/0627 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/12 (2013.01); B01L 2300/165 (2013.01); B01L 2300/168 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An apparatus, comprising:
a first layer defining a first opening, a second opening, and a microfluidic channel that establishes a fluid communication path between the first opening and the second opening, the first layer being substantially transparent;
a second layer coupled to the first layer, and the second layer being partially opaque,
wherein the first layer is a top portion of the apparatus and the second layer is a bottom portion of the apparatus;
wherein the first opening is larger than the second opening and is configured to receive a fluid, and the second opening is configured to vent air out of the apparatus as the microfluidic channel is filled with the fluid;
wherein a height of the microfluidic channel decreases continuously from the first opening to the second opening, or a side of the microfluidic channel formed in the first layer defines a set of steps such that a height of the microfluidic channel decreases in a step-wise manner from the first opening to the second opening; and
wherein reagents including at least one of lysing agents and contrast agents are disposed throughout a length of the microfluidic channel.