US 11,654,378 B2
Senna obtusifolia seed extract and a method for comprehensive development and utilization of Senna obtusifolia seeds
Sujing Gu, Luohe (CN); Ziheng Jin, Luohe (CN); Yanjun Wen, Luohe (CN); and Linzheng Li, Luohe (CN)
Filed by Sujing Gu, Luohe (CN); Ziheng Jin, Luohe (CN); Yanjun Wen, Luohe (CN); and Linzheng Li, Luohe (CN)
Filed on Jun. 19, 2021, as Appl. No. 17/352,295.
Claims priority of application No. 202010616852.9 (CN), filed on Jun. 30, 2020.
Prior Publication US 2021/0402324 A1, Dec. 30, 2021
Int. Cl. B01D 11/02 (2006.01); C07C 50/18 (2006.01); C08B 37/08 (2006.01); B01D 11/04 (2006.01); B01D 61/14 (2006.01); C08B 37/00 (2006.01)
CPC B01D 11/0288 (2013.01) [B01D 11/0284 (2013.01); B01D 11/0415 (2013.01); B01D 11/0492 (2013.01); B01D 61/145 (2013.01); C07C 50/18 (2013.01); C08B 37/0087 (2013.01)] 10 Claims
1. A method of preparing a Senna obtusifolia seed extract rich in anthraquinones and a galactomannan extract, comprising the following steps sequentially:
(1) crushing Senna obtusifolia seeds into a Senna obtusifolia seed powder;
(2) extracting the Senna obtusifolia seed powder with 40-85% aqueous ethanol solution, filtering to obtain an extract solution and a residue;
(3) concentrating the extract solution under vacuum to obtain a concentrated extract solution, spray-drying the concentrated extract solution to obtain the Senna obtusifolia seed extract;
(4) extracting the residue with membrane filtered water, conducting a centrifugation to obtain a supernatant;
(5) adding ammonium sulfate and ethanol to the supernatant to form a two-phase aqueous system, collecting a bottom layer of the two-phase aqueous system; and
(6) conducting an ultrafiltration of the bottom layer with a cut-off molecular weight of 50 k-200 k to obtain a galactomannan extract solution, drying the galactomannan extract solution under vacuum to obtain the galactomannan extract.