US 11,654,161 B2
Compositions and methods for neuralgenesis
Thomas Hartung, Baltimore, MD (US); David Pamies, Baltimore, MD (US); and Helena T. Hogberg, Nottingham, MD (US)
Assigned to The Johns Hopkins University, Baltimore, MD (US)
Filed by The Johns Hopkins University, Baltimore, MD (US)
Filed on Dec. 2, 2019, as Appl. No. 16/700,750.
Application 16/700,750 is a continuation of application No. 16/077,411, previously published as PCT/US2017/017464, filed on Feb. 10, 2017.
Claims priority of provisional application 62/294,112, filed on Feb. 11, 2016.
Prior Publication US 2020/0121725 A1, Apr. 23, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 35/30 (2015.01); A61K 38/41 (2006.01); C12N 5/074 (2010.01); G01N 33/68 (2006.01); G01N 33/50 (2006.01); C12N 5/00 (2006.01); G01N 33/58 (2006.01)
CPC A61K 35/30 (2013.01) [A61K 38/41 (2013.01); C12N 5/0062 (2013.01); C12N 5/0696 (2013.01); G01N 33/50 (2013.01); G01N 33/58 (2013.01); G01N 33/6893 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2501/13 (2013.01); C12N 2501/22 (2013.01)] 22 Claims
 
1. An in vitro brain microphysiological system (BMPS), comprising
A. an induced pluripotent stem cell (iPSC) wherein the iPSC is configured to differentiate into at least one neural cell type aggregated into a spheroid mass; and a population of microglia-like cells, wherein
B. the in vitro BMPS is electrophysiologically active in a spontaneous manner.