US 11,653,874 B2
Method and system for characterizing tissue in three dimensions using multimode optical measurements
Daniel L. Farkas, Beverly Hills, CA (US); Fartash Vasefi, Sherman Oaks, CA (US); and Nicholas MacKinnon, Vancouver (CA)
Assigned to ACCELERITAS CORPORATION, Sherman Oaks, CA (US)
Filed by ACCELERITAS CORPORATION, Sherman Oaks, CA (US)
Filed on Aug. 7, 2015, as Appl. No. 14/821,672.
Application 14/821,672 is a continuation in part of application No. 14/815,691, filed on Jul. 31, 2015, abandoned.
Application 14/815,691 is a continuation of application No. PCT/US2014/014330, filed on Jan. 31, 2014.
Claims priority of provisional application 61/759,910, filed on Feb. 1, 2013.
Prior Publication US 2015/0374276 A1, Dec. 31, 2015
Int. Cl. A61B 5/00 (2006.01); A61B 5/1455 (2006.01); A61B 5/145 (2006.01)
CPC A61B 5/443 (2013.01) [A61B 5/14558 (2013.01); A61B 5/444 (2013.01); A61B 5/14546 (2013.01); A61B 5/14551 (2013.01); A61B 2576/02 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method of characterizing biological tissue, comprising:
illuminating tissue in vivo with a temporal sequence of multiple wavelengths of polarized light comprising a continuum between 400 nm and 1000 nm;
separating light emitted from said tissue in response to said illumination into at least two polarization components, wherein the at least two polarization components comprise different linear polarizations of the separated light, and are captured using parallel and cross orientations of a polarizing filter in a detection path with respect to an orientation of a second polarizing filter in an illumination path used to illuminate the tissue;
calculating a degree of linear polarization of the light emitted from said tissue based on measurements of an intensity for each of the at least two polarization components of the emitted light, respectively;
forming at least two respective hyperspectral image sets from said at least two polarization components;
identifying a distribution of superficial melanin in an illuminated portion of the tissue and cancelling a contribution of the superficial melanin in one or more images in the hyperspectral image sets, and thereafter estimating an amount of oxyhemoglobin, deoxyhemoglobin, or both oxyhemoglobin and deoxyhemoglobin, in the tissue, wherein superficial melanin is melanin between the surface of the skin and 120 μm depth;
generating a three-dimensional model of the tissue comprising an array of individual tissue-characteristic three-dimensional voxels based on the degree of linear polarization of the emitted light as a function of wavelength and position in measurement space; and
determining at least one characteristic of said tissue.