US 11,653,672 B2
Gas fermentation for the production of protein or feed
Sean Simpson, Evanston, IL (US); Wyatt Eric Allen, Chicago, IL (US); Robert John Conrado, Washington, DC (US); and Sean Molloy, Auckland (NZ)
Assigned to LanzaTech NZ, Inc., Skokie, IL (US)
Filed by LanzaTech NZ, Inc., Skokie, IL (US)
Filed on Nov. 20, 2020, as Appl. No. 17/100,640.
Application 17/100,640 is a continuation of application No. 15/159,479, filed on May 19, 2016, granted, now 10,856,560, issued on Dec. 8, 2020.
Claims priority of provisional application 62/165,182, filed on May 21, 2015.
Prior Publication US 2021/0084933 A1, Mar. 25, 2021
Int. Cl. A23K 10/12 (2016.01); C12N 1/20 (2006.01); A23K 50/10 (2016.01); A23K 50/40 (2016.01); A23K 50/30 (2016.01)
CPC A23K 10/12 (2016.05) [A23K 50/10 (2016.05); A23K 50/30 (2016.05); A23K 50/40 (2016.05); C12N 1/20 (2013.01)] 16 Claims
1. A process comprising:
a. obtaining a gaseous substrate comprising one or more of CO, or CO2, and H2 from an industrial process waste gas, an industrial process off gas, or syngas;
b. culturing the gaseous substrate in a two reactor system by contacting it with a microorganism, which is an anaerobe, selected from the group consisting of Acetobacterium woodii, Alkalibaculum bacchii, Blautia producta, Butyribacterium methylotrophicum, Clostridium aceticum, Clostridium autoethanogenum, Clostridium carboxidivorans, Clostridium coskatii, Clostridium drakei, Clostridium formicoaceticum, Clostridium ljungdahlii, Clostridium magnum, Clostridium ragsdalei, Clostridium scatologenes, Eubacterium limosum, Moorella thermautotrophica, Moorella thermoacetica, Oxobacter pfennigii, Sporomusa ovata, Sporomusa silvacetica, Sporomusa sphaeroides, Thermoanaerobacter kivui, and mixtures thereof, under fermentation conditions wherein the microorganism dilution rate in the second reactor is higher than the microorganism dilution rate in the first reactor, and the microorganism dilution rate of the second reactor is from about 1/day to about 2/day to maximize production of microbial biomass, and to generate a product selected from the group consisting of acetate, acetic acid, ethanol, 2,3-butanediol, lactic acid, and mixtures thereof, in a fermentation broth;
c. isolating the biomass from the fermentation broth,
d. drying the microbial biomass wherein the microbial biomass contains greater than 85% protein by weight on a dry solids basis,
e. separating at least one product from at least a portion of the fermentation broth.