US 11,653,636 B2
Method of making a rat model of retinal degeneration and rat model made thereby
Joo Yong Lee, Seoul (KR); In Jeoung Baek, Seoul (KR); and Young Hoon Sung, Seoul (KR)
Assigned to UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRYCOOPERATION, Ulsan (KR); and THE ASAN FOUNDATION, Seoul (KR)
Filed by UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRY COOPERATION, Ulsan (KR); and THE ASAN FOUNDATION, Seoul (KR)
Filed on Nov. 15, 2019, as Appl. No. 16/686,024.
Prior Publication US 2021/0144976 A1, May 20, 2021
Int. Cl. C12N 15/00 (2006.01); A01K 67/027 (2006.01); G01N 33/00 (2006.01); A61K 49/00 (2006.01); C12N 15/85 (2006.01)
CPC A01K 67/0276 (2013.01) [A61K 49/0008 (2013.01); C12N 15/8509 (2013.01); A01K 2217/075 (2013.01); A01K 2227/105 (2013.01); A01K 2267/0306 (2013.01); C12N 2015/8536 (2013.01)] 10 Claims
 
1. A method for producing a genetically modified rat model of retinal degeneration, the method comprising:
a) generating CRISPR RNA (crRNA) that targets a rat PDE6B gene and mRNA encoding CPF1 endonuclease;
b) injecting the crRNA and mRNA into a rat embryo; and
c) implanting the embryo into a foster mother such that a rat comprising germline cells whose genomes comprise an inactivated PDE6B gene is obtained;
d) crossing the rat obtained in step c) with a wild-type rat such that a genetically modified rat whose genome comprises a heterozygous inactivated PDE6B gene is obtained;
e) crossing the rat obtained in step d) with a littermate such that a genetically modified rat whose genome comprises a homozygous inactivated PDE6B gene with increased susceptibility to retinal degeneration is obtained.