US 11,653,609 B2
Generating maize plants with enhanced resistance to northern leaf blight
Zhenglin Hou, Ankeny, IA (US); April L Leonard, Des Moines, IA (US); Bailin Li, Johnston, IA (US); and Girma M Tabor, Johnston, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC.
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed on Jun. 17, 2020, as Appl. No. 16/903,814.
Application 16/903,814 is a continuation of application No. 15/765,566, abandoned, previously published as PCT/US2016/057081, filed on Oct. 14, 2016.
Claims priority of provisional application 62/242,691, filed on Oct. 16, 2015.
Prior Publication US 2021/0000059 A1, Jan. 7, 2021
Int. Cl. C12N 15/82 (2006.01); A01H 6/46 (2018.01); C07K 14/415 (2006.01)
CPC A01H 6/4684 (2018.05) [C07K 14/415 (2013.01); C12N 15/8282 (2013.01)] 19 Claims
 
1. A maize plant cell comprising a heterologous polynucleotide construct comprising an isolated polynucleotide operably linked to a regulatory element, wherein the isolated polynucleotide comprises:
a. the nucleotide sequence set forth in SEQ ID NO:1 (PH4GP c-DNA), SEQ ID NO:3 (PH1W2 cDNA), or SEQ ID NO:9 (PH4GP Genomic sequence);
b. a nucleotide sequence encoding a CC-NB-LRR polypeptide comprising an amino acid sequence of at least 95% sequence identity to SEQ ID NO:2 or SEQ ID NO:4, wherein the CC-NB-LRR polypeptide comprises SEQ ID NO:10 at the positions corresponding to amino acids 633-648 of SEQ ID NO:2 or SEQID NO:4, respectively; or
c. a nucleotide sequence encoding a CC-NB-LRR polypeptide comprising the sequence set forth in SEQ ID NO:2 or SEQ ID NO:4.