US 11,653,604 B2
Cross pollination through liquid-mediated delivery of pollen to enclosed stigmas of flowers from recipient plants
Huachun Wang Larue, Chesterfield, MO (US); and Li Lin, St. Louis, MO (US)
Assigned to MONSANTO TECHNOLOGY LLC, St. Louis, MO (US)
Filed by MONSANTO TECHNOLOGY LLC, St. Louis, MO (US)
Filed on Oct. 1, 2019, as Appl. No. 16/589,926.
Prior Publication US 2021/0092920 A1, Apr. 1, 2021
Int. Cl. A01H 1/02 (2006.01); C12Q 1/6876 (2018.01)
CPC A01H 1/02 (2013.01) [C12Q 1/6876 (2013.01); C12Q 2600/158 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method for liquid-mediated delivery of pollen to an enclosed stigma of a flower from a recipient soybean plant comprising the steps of:
a) obtaining pollen from a donor soybean plant;
b) producing a liquid solution comprising said pollen; and
c) injecting said solution into an enclosed stigma of an unopened flower bud on a recipient soybean plant, thereby pollinating the flower bud with the pollen from the donor soybean plant, wherein the enclosed stigma is enclosed by flower structures.