US 12,308,095 B1
Prediction of computational pathway circuits
Clifford Anders Olson, Long Beach, CA (US); Kayvan Niazi, Agoura Hills, CA (US); Nicholas J. Witchey, Laguna Hills, CA (US); and Wael Tadros, Los Angeles, CA (US)
Assigned to NantBio, Inc., Culver City, CA (US)
Filed by NantBio, Inc., Culver City, CA (US)
Filed on Jun. 3, 2020, as Appl. No. 16/891,935.
Claims priority of provisional application 62/860,142, filed on Jun. 11, 2019.
Int. Cl. G16B 5/00 (2019.01); G16B 25/10 (2019.01)
CPC G16B 5/00 (2019.02) [G16B 25/10 (2019.02)] 8 Claims
 
1. A method for making a recombinant cell, the method comprising:
(i) generating, in silico via PARADIGM, a pathway map of an endogenous IL-8 signaling pathway and a HIF-1a signaling pathway in a cell of interest;
(ii) determining, in silico, an expected effect of incorporating a recombinant nucleic acid on pathways of interest within the cell, wherein the recombinant nucleic acid comprises a promotor that is activated upon sensing IL-8 and HIF-1a signaling, and wherein the promotor controls expression of a sequence encoding a therapeutic molecule; and
(iii) transfecting the cell of interest with the recombinant nucleic acid, thereby making a recombinant cell that expresses the therapeutic molecule only upon IL-8 and HIF-1a signaling.