US 12,307,670 B1
Analyzing image data from nucleic acid sequencing
Francisco Jose Garcia, San Diego, CA (US); Klaus Maisinger, Essex (GB); Stephen Tanner, San Diego, CA (US); John A. Moon, San Diego, CA (US); Tobias Mann, San Diego, CA (US); Michael Lawrence Parkinson, Reyrieux (FR); Anthony James Cox, Essex (GB); and Haifang H. Ge, Essex (GB)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on Jan. 8, 2025, as Appl. No. 19/013,541.
Application 19/013,541 is a continuation of application No. 18/303,436, filed on Apr. 19, 2023, granted, now 12,223,651.
Application 18/303,436 is a continuation of application No. 17/445,994, filed on Aug. 26, 2021, granted, now 11,676,275, issued on Jun. 13, 2023.
Application 17/445,994 is a continuation of application No. 17/157,622, filed on Jan. 25, 2021, granted, now 11,605,165, issued on Mar. 14, 2023.
Application 17/157,622 is a continuation of application No. 16/378,894, filed on Apr. 9, 2019, abandoned.
Application 16/378,894 is a continuation of application No. 15/354,540, filed on Nov. 17, 2016, granted, now 10,304,189, issued on May 28, 2019.
Application 15/354,540 is a continuation of application No. 14/608,471, filed on Jan. 29, 2015, granted, now 9,530,207, issued on Dec. 27, 2016.
Application 14/608,471 is a continuation of application No. 13/006,206, filed on Jan. 13, 2011, granted, now 8,965,076, issued on Feb. 24, 2015.
Claims priority of provisional application 61/321,029, filed on Apr. 5, 2010.
Claims priority of provisional application 61/294,811, filed on Jan. 13, 2010.
Int. Cl. G06T 7/00 (2017.01); G06T 7/246 (2017.01); G06T 7/33 (2017.01); G06T 7/90 (2017.01)
CPC G06T 7/0014 (2013.01) [G06T 7/0012 (2013.01); G06T 7/246 (2017.01); G06T 7/248 (2017.01); G06T 7/33 (2017.01); G06T 7/337 (2017.01); G06T 7/90 (2017.01); G06T 2207/10024 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30072 (2013.01)] 30 Claims
OG exemplary drawing
 
1. A method for analysis of image data generated during nucleic acid sequencing:
obtaining fluorescent emission signals from nucleic acid sites on a flow cell, wherein a nucleic acid site comprises multiple copies of a nucleic acid, and wherein the fluorescent emission signals are indicative of nucleotides present at the nucleic acid site;
identifying candidate site locations on the flow cell from the fluorescent emission signals, the fluorescent emission signals detected by different detection channels;
determining the quality of the identified candidate site locations, wherein the quality of a candidate site location is determined based on relative intensities of fluorescent emission signals detected at the identified candidate site location by the different detection channels; and
generating a template of nucleic acid site locations from the candidate site locations based on the quality of the identified candidate site locations, wherein identified candidate site locations that do not meet a threshold level of quality are excluded from the template.